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KMID : 0614020060210010025
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Journal of Pharmaceutical Sciences (C.N.U.)
2006 Volume.21 No. 1 p.25 ~ p.29
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Lectin activities of Korean basidiomycetes including Psathyrella velutina
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Chung Kyeong-Soo
Chung Youn-Ju
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Abstract
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As an effort to develop noble antitumor lectins from Korean basidiomycetes, wild mushrooms were collected and their cold-water extracts were subjected to a screening test for their lectin activities using hemmagglutination assay on BALB/c mouse erythrocytes. Of the nine species tested, three species, Psathyrella velutina, Fomitella fraxinea and Russula compacta, showed strong hemmagglutinating activities, while the other six species showed minimal or no activities. Of these, partial purification of the lectin components of P. velutina and R. compacta were attempted by precipitation with saturated ammonium sulfate followed strong hemmagglutination activity unlike that of R. compacta, which lost its lectin activity after the purification process. Moreover, the partially purified P. velutina lectin exerted cytotoxic effect on sarcoma 180 cells when assessed by flow cytometric analysis.
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KEYWORD
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lectin, asidiomycetes, mushroom, hemmagglutination, Psathyrella velutina, Fomitella fraxinea, Russula compacta, cytotoxic, flow cytometric analysis
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